For USB boot mode
Target Board: EK-RA2A1

Steps to follow:
Step1: USB FS is used for this mode, Connect Device USB (J9) on EK-RA2A1 board.
Step2: Power the board using Debug USB or external Power.
Step3: Press the RESET button(S2), once done you can find the comport detected.
Step4: Open the RFP tool and create project as below, In Tool select COM Port, COM 12 detected automatically(verify in ports RA USB Boot (CDC) (Com 12) ).
Step5: Once Project is successfully created, Browse the .SREC file in debug folder of your project for eg: E:\MOHAN ..PATH \RA2L1\Workspace\EK_RA2A1_LED\Debug\EK_RA2A1_LED.srec

STEP6: Again press RESET button S2, and give Start, Once Completed you will find like below (Refer attached docx file).
STEP7: Do a power cycle, so that the application will be running.

Note: Procedure attached with snapshots.